PTM Viewer

For each protein all PTMs are highlighted by default in the respective protein sequence (right-hand side). One can adjust a selection of PTMs in the PTM table on the left-hand side. In addition, functional protein domains and sites can be underlined if desired.

In the PTM table per PTM the PTM position and type is indicated, as well as the plain peptide sequence that was identified by mass spectrometry. The respective proteomics study is indicated by a number, providing a link to consult the experimental details. Additional PTM meta-data includes various confidence measures such as peptide score provided by search algorithms, posterior error probability (PEP), precursor mass deviation (in ppm) and modification site probability. The available confidence meta-data can be consulted in the extended PTM table by clicking SHOW CONFIDENCE. However, in the default PTM table, a color-coding of confidence is provided with green indicating high confidence, olive medium confidence, grey low confidence, and no color an unassigned confidence. More details regarding this confidence assignment can be consulted in the tutorial or the Plant PTM Viewer manuscript.

Besides confidence measures, log2 fold changes between two conditions with significance values (P- or Q-values) are shown if provided in the respective publication. Log2 fold changes are colored in heatmap-like gradient (green = induced, red = repressed) and significant values are highlighted in green. To determine significance, we employed the threshold used in the respective publication. For more details on the quantitative measurements we refer to the experimental details and respective publication, as methodologies can differ.

On the bottom of the page one can send the whole protein or a part of the protein (i.e. a functional domain) to PTM Blast. This will display aligned protein sequences that potentially report aligned PTMs.

PTMs

Export
Results

PTM Type	Mod AA	Pos	Peptide	Exp ID	Log2 FC	P/Q val	Score Type	Ident Score	Loc Prob	PEP	Mass Error (ppm)
ph	S	9	RASEVPTK	114			MaxQuant score	138.760	1.000	1.816e-3
sno	C	102	LVLENCTNK	169	-0.172	0.047
ph	S	257	AFSSLATR	114			MaxQuant score	107.550	0.997	3.920e-3
ox	C	361	AIDSVICNTSK	138a	1.030	0.042
sno	C	361	AIDSVICNTSK	169	-0.315	0.254
ph	S	893	SVDRASDNSVSASSSTAER	114			MaxQuant score	73.649	0.981	5.720e-5
ph	S	896	ASDNSVSASSSTAER	114			MaxQuant score	94.454	0.997	4.282e-5
ph	S	902	ASDNSVSASSSTAERESDEDSSNALAVR	83			MASCOT Score	72.000			0.000
ph	T	903	ASDNSVSASSSTAERESDEDSSNALAVR	48			MASCOT Score	58.200
ph	S	908	ASDNSVSASSSTAERESDEDSSNALAVR	48			MASCOT Score	46.630
				83			MASCOT Score	96.000			0.001
				84a			XCorr	5.130	0.989	1.930e-11
				85			XCorr	5.162	0.997	1.785e-4
				88			MASCOT Score	45.000	1.000		0.570
				109					0.982
				111a	0.347	0.000		60.020			1.338
				111b	0.347	0.000		60.020			1.338
				111c	0.045	0.000		60.020			1.338
				111d	0.045	0.000		60.020			1.338
			ESDEDSSNALAVR	114			MaxQuant score	269.900	1.000	0.000e+0
ph	S	912	ESDEDSSNALAVR	114			MaxQuant score	190.340	0.961	3.252e-30
ph	S	925	YTNPVSIR	114			MaxQuant score	112.130	1.000	3.534e-3
ph	S	930	SSSSQSIWGGHR	114			MaxQuant score	157.030	1.000	9.554e-10
ph	S	931	SSSSQSIWGGHR	114			MaxQuant score	81.619	0.837	2.804e-4
ph	S	933	SSSSQSIWGGHR	114			MaxQuant score	137.390	0.998	1.737e-5
ph	S	943	EFLSVVR	114			MaxQuant score	93.649	1.000	1.444e-2
ph	S	973	HLESFNFDSEIPADLPVTSSSHELK	114			MaxQuant score	151.370	1.000	1.962e-44
ph	S	989	HLESFNFDSEIPADLPVTSSSHELK	48			MASCOT Score	33.090
				111a	0.698	0.000		47.340			-0.088
				111b	0.698	0.000		47.340			-0.088
				111c	1.725	0.000		47.340			-0.088
				111d	1.725	0.000		47.340			-0.088
				114			MaxQuant score	220.630	0.769	0.000e+0
sno	C	1011	LNCTLR	169
ub	K	1152	NLWSHSKWLVDTLQNYCR	124
ph	S	1524	DALSSDVAK	88			MASCOT Score	44.000	0.959		-1.330
ph	S	1524	DALSSDVAK	114			MaxQuant score	68.536	0.796	1.172e-2
ph	S	1525	DALSSDVAK	114			MaxQuant score	78.513	0.931	5.212e-3
ph	S	1703	VSGSEHGFSLNEPLGISENK	106			MASCOT Score	23.000
				109					0.844
				114			MaxQuant score	296.530	0.952	0.000e+0
ph	S	1705	VSGSEHGFSLNEPLGISENK	48			MASCOT Score	58.560
				83			MASCOT Score	60.000			-0.000
				85			XCorr	4.190	0.933	1.612e-9
				88			MASCOT Score	29.000	0.993		-0.650
				106	0.098	0.749	MASCOT Score	39.000
				109					0.928
				111a	0.852	0.039		88.170			0.986
				111b	0.852	0.039		88.170			0.986
				111c	1.076	0.505		88.170			0.986
				111d	1.076	0.505		88.170			0.986
				114			MaxQuant score	494.760	1.000	0.000e+0
ph	S	1710	VSGSEHGFSLNEPLGISENK	83			MASCOT Score	51.000			0.001
				84a			XCorr	4.630	0.879	1.280e-6
				84b			XCorr	5.040	0.932	6.660e-16
				106	0.099	0.747	MASCOT Score	25.000
				111a	3.106	0.000		49.720			0.372
				111b	3.106	0.000		49.720			0.372
				111c	0.145	0.000		49.720			0.372
				111d	0.145	0.000		49.720			0.372
				114			MaxQuant score	83.701	0.952	1.397e-9
ph	S	1844	GSNLPDDSPGNGGLIYHVIHR	114			MaxQuant score	157.900	1.000	6.069e-30
ph	S	1917	SSSQSVLLPDKR	114			MaxQuant score	54.315	0.769	4.975e-3
sno	C	1950	NSSSSNFPGCGCSPDVAK	169
ph	T	2018	SEVPNEQKNTDSDER	114			MaxQuant score	76.027	0.959	1.502e-4
ph	S	2072	GSSLTQAMLQEMR	114			MaxQuant score	204.130	0.998	9.677e-39
ph	S	2111	EEIEGDQIEMSFHVEDR	114			MaxQuant score	125.770	1.000	3.615e-10
ph	S	2278	QTGRSSFDR	114			MaxQuant score	84.169	0.880	8.566e-4
ph	S	2285	SGSEVHGFQHPLFSR	88			MASCOT Score	61.000	0.895		-0.310
				109					0.922
				114			MaxQuant score	180.080	0.999	0.000e+0
ph	S	2306	PSQTGNTASVSASAGSISR	114			MaxQuant score	207.370	0.988	0.000e+0
ph	S	2308	PSQTGNTASVSASAGSISR	88			MASCOT Score	38.000	0.907		0.480
ph	S	2310	PSQTGNTASVSASAGSISR	88			MASCOT Score	68.000	1.000		0.040
ph	S	2313	PSQTGNTASVSASAGSISR	88			MASCOT Score	68.000	0.999		0.040
ph	S	2313	PSQTGNTASVSASAGSISR	114			MaxQuant score	324.230	0.995	0.000e+0
ph	S	2315	PSQTGNTASVSASAGSISR	88			MASCOT Score	54.000	0.995		0.020
ph	S	2363	LGGGGAPPPLTDYSVVGMDSSR	114			MaxQuant score	119.380	0.975	2.495e-21
ph	S	2582	NDEQVDEGSLDGRAPEVNAIDPTFLEALPEDLR	85			XCorr	5.020	1.000	4.542e-4
			NDEQVDEGSLDGRAPEVNAIDPTFLEALPEDLR	100			XCorr	4.700	1.000	2.574e-4
			NDEQVDEGSLDGR	38			MASCOT Score	93.910			-1.061
				59			MASCOT Score	85.000			-0.793
				60			MASCOT Score	85.250			-2.777
				84a			XCorr	4.450	1.000	7.320e-6
				84b	1.563		XCorr	4.650	1.000	6.950e-14
				85			XCorr	4.018	1.000	2.778e-2
				88			MASCOT Score	69.000	1.000		-0.120
				100			XCorr	4.340	1.000	3.325e-7
				106	0.154	0.581	MASCOT Score	44.000
				109					1.000
				111a	-1.416	0.000		70.410			0.998
				111b	-1.416	0.000		70.410			0.998
				111c	-1.698	0.000		70.410			0.998
				111d	-1.698	0.000		70.410			0.998
				114			MaxQuant score	321.090	1.000	0.000e+0
ph	S	2724	VFGSSHR	114			MaxQuant score	88.819	0.827	1.811e-2
ph	S	2725	VFGSSHR	114			MaxQuant score	102.620	0.867	1.811e-2
ph	S	2756	DVSSSADGLK	88			MASCOT Score	64.000	1.000		-1.630
ph	S	2756	DVSSSADGLK	114			MaxQuant score	90.653	0.980	1.202e-3
ph	S	2757	DVSSSADGLK	114			MaxQuant score	77.541	0.799	3.578e-3
ph	S	2758	DVSSSADGLK	88			MASCOT Score	38.000	0.998		-1.820
				109					0.795
				114			MaxQuant score	113.950	0.997	9.501e-5
ph	S	2977	LENKPVGEEASSETQKDAESELSVAR	114			MaxQuant score	177.860	0.764	0.000e+0
ph	S	2978	LENKPVGEEASSETQK	100			XCorr	4.733	0.999	8.060e-5
ph	S	2978	LENKPVGEEASSETQK	114			MaxQuant score	167.900	0.940	8.063e-19
ph	T	2980	LENKPVGEEASSETQKDAESELSVAR	114			MaxQuant score	92.761	0.944	6.710e-15
ph	T	2980	LENKPVGEEASSETQK	100			XCorr	2.810	0.990	2.226e-3
ph	S	2986	DAESELSVAR	59			MASCOT Score	55.000			-0.865
				83			MASCOT Score	33.000			0.001
				88			MASCOT Score	54.000	1.000		-0.120
				100			XCorr	2.418	0.999	6.727e-4
				114			MaxQuant score	183.990	1.000	2.337e-28
ph	S	2989	DAESELSVAR	43			MASCOT Score	56.380			-0.406
ph	S	2989	DAESELSVAR	114			MaxQuant score	169.890	1.000	2.736e-10
ph	S	3317	MRSPQDLK	88			MASCOT Score	27.000	1.000		-1.800
ph	S	3317	MRSPQDLK	114			MaxQuant score	82.452	1.000	1.558e-2
ub	K	3589	FLQFVTGTSKVPLEGFK	168	-0.302	0.560	MaxQuant score	68.845	1.000	7.631e-5	-2.108
ph	S	3615	AYGSPERLPSAHTCFNQLDLPEYQSK	114			MaxQuant score	288.040	1.000	0.000e+0
so	C	3625	LPSAHTCFNQLDLPEYQSK	108			pFind PSM Score	0.096			-0.431

Sequence

Length: 3658

MKLRRRRASEVPTKISLFINSVTSVPLELIQEPLASFRWEFDKGDFHHWVDLFYHFDTFFEKHVKVRKDLRIEEEFDESDPPFPKDAVLQVLRVIRLVLENCTNKQFYTSYEQHLSLLLASTDADVVEACLQTLAAFLKRPTGKYSIRDASLNLKLFSLAQGWGGKEEGLGLTSCATEHSCDQLFLQLGCTLLFEFYASDESPSELPGGLQVIHVPDVSMRSESDLELLNKLVIDHNVPPSLRFALLTRLRFARAFSSLATRQQYTCIRLYAFIVLVQASGDTENVVSFFNGEPEFVNELVTLVSYEDTVPAKIRILCLQSLVALSQDRTRQPTVLTAVTSGGHRGLLSGLMQKAIDSVICNTSKWSLAFAEALLSLVTVLVSSSSGCSAMREAGLIPTLVPLIKDTDPQHLHLVSTAVHILEVFMDYSNPAAALFRDLGGLDDTIFRLKQEVSRTEDDVKEIVCCSGSNGPEDDTEQLPYSEALISYHRRLLLKALLRAISLGTYAPGNTNLYGSEESLLPECLCIIFRRAKDFGGGVFSLAATVMSDLIHKDPTCFNALDSAGLTSAFLDAISDEVICSAEAITCIPQCLDALCLNNSGLQAVKDRNALRCFVKIFSSPSYLKALTSDTPGSLSSGLDELLRHQSSLRTYGVDMFIEILNSILIIGSGMEATTSKSADVPTDAAPVPMEIDVDEKSLAVSDEAEPSSDTSPANIELFLPDCVCNVARLFETVLQNAEVCSLFVEKKGIDTVLQLFSLPLMPLSTSLGQSFSVAFKNFSPQHSAGLARILCSYLREHLKKTNNLLVSIEGTQLLKLESAVQTKILRSLSCLEGMLSLSNFLLKGSASVISELSAANADVLKELGITYKQTIWQMALCNDTKEDEKKSVDRASDNSVSASSSTAERESDEDSSNALAVRYTNPVSIRSSSSQSIWGGHREFLSVVRSGRGVHGHTRHAIARMRGGRTRRHLESFNFDSEIPADLPVTSSSHELKKKSTEVLIAEILNKLNCTLRFFFTSLVKGFTSANRRRIDGPSLSSASKTLGTALAKVFLEALNFQGYGAAAGPDTSLSLKCRYLGKVVDDITFLTFDTRRRVCFTAMVNSFYVHGTFKELLTTFEATSQLLWKVPFSIRASSTENEKSGERNLWSHSKWLVDTLQNYCRALDYFVNSTYLLSPTSQTQLLVQPASVDLSIGLFPVPREPETFVRNLQSQVLEVILPIWNHPMFPDCNPNFVASVTSLVTHIYSGVVDTRENRSGATQGTNQRALPLQPDEAIVGMIVEMGFSRSRAEDALRRVGTNSVEMAMDWLFTNPEDPVQEDDELAQALALSLGNSSETPKLEDTEKPVDVPQEEAEPKEPPVDEVIAASVKLFQSDDSIAFPLVDLFVTLCNRNKGEDRPKIVFYLIQQLKLVQLDFSKDTGALTMIPHILALVLSEDDNTREIAAQDGIVAVAIGILTDFNLKSESETDILAPKCISALLLVLSMMLQAQTRLSSEYVEGNQGGSLVLSDSPQDSTAALKDALSSDVAKGESNQALESMFGKSTGYLTMEESSKVLLIACGLIKQRVPAMIMQAVLQLCARLTKSHALAIQFLENGGLSSLFNLPKKCFFPGYDTVASVIVRHLVEDPQTLQIAMETEIRQTLSGKRHIGRVLPRTFLTTMAPVISRDPVVFMKAVASTCQLESSGGTDFVILTKEKEKPKVSGSEHGFSLNEPLGISENKLHDGSGKCSKSHRRVPTNFIQVIDQLIDIVLSFPGLKRQEGEAANLISMDVDEPTTKVKGKSKVGEPEKAELGSEKSEELARVTFILKLLSDIVLMYLHGTSVILRRDTEISQLRGSNLPDDSPGNGGLIYHVIHRLLPISLEKFVGPEEWKEKLSEKASWFLVVLCSRSNEGRKRIINELTRVLSVFASLGRSSSQSVLLPDKRVLAFANLVYSILTKNSSSSNFPGCGCSPDVAKSMIDGGTIQCLTSILNVIDLDHPDAPKLVTLILKSLETLTRAANAAEQLKSEVPNEQKNTDSDERHDSHGTSTSTEVDELNQNNSSLQQVTDAVDNGQEQPQVSSQSEGERGSSLTQAMLQEMRIEGDETILPEPIQMDFFREEIEGDQIEMSFHVEDRADDDVDDDMDDEGEDDEGDDEDADSVEDGAGVMSIAGTDVEDPEDTGLGDEYNDDMVDEDEEDEDEYNDDMVDEDEDDEDEYNDDMVDEDEDDFHETRVIEVRWREALDGLDHFQIVGRSGGGNGFIDDITAEPFEGVNVDDLFALRRSLGFERRRQTGRSSFDRSGSEVHGFQHPLFSRPSQTGNTASVSASAGSISRHSEAGSYDVAQFYMFDSPVLPFDQVPVDPFSDRLGGGGAPPPLTDYSVVGMDSSRRGVGDSRWTDVGHPQPSSLSASIAQLIEEHFITNLRASAPVDTVVERETNTTEVQEQQQPDVPPSVGSETVLGDGNEGGEQSEEHELLNNNEVMHPLPLNSTPNEIDRMEVGEGGGAPIEQVDREAVHLISSAQGQSDTSGIQNVSVTAIPPPVDDPDSNFQPSVDVDMSSDGAEGNQSVQPSPLDGDNNELSSMEATQDVRNDEQVDEGSLDGRAPEVNAIDPTFLEALPEDLRAEVLASQQAQSVQPPTYEPPSVDDIDPEFLAALPPEIQREVLAQQRAQRMLQQSQGQPVDMDNASIIATLPADLREEVLLTSSEAVLAALPPPLLAEAQMLRDRAMRHYQARSRVFGSSHRLNNRRNGLGYRLTGMERGVGVTIGQRDVSSSADGLKVKEIEGDPLVNADALKSLIRLLRLAQPLGKGLLQRLLLNLCAHSFTRANLVQLLLDMIRPEMETLPSELALTNPQRLYGCQLNVVYGRSQLLNGLPPLVFRRVLEVLTYLATNHSAVADMLFYFDSSLLSQLSSRKGKEKVTHETDSRDLEIPLVVFLKLLNRPQLLQSTSHLALVMGLLQVVVYTAASRIEGWSPSSGVPEKLENKPVGEEASSETQKDAESELSVARRKNCAELYNIFLQLPQSDLCNLCMLLGYEGLSDKIYSLAGEVLKKLAAVDVTHRKFFTKELSELASGLSSSTVRVLATLSTTQKMSQNTCSMAGASILRVLQVLSSLTSTIDDSNVGTDKETDQEEQNIMQGLKVALEPLWQELGQCISMTELQLDHTAATSNVNPGDHVLGISPTSSLSPGTQSLLPLIEAFFVLCEKIQTPSMLQQDATVTAGEVKESSTHGSSSKTIVDSQKKIDGSVTFSKFVEKHRRLLNSFVRQNPSLLEKSFSMMLKAPRLIDFDNKKAYFRSRIRHQHDQHISGPLRISVRRAYVLEDSYNQLRMRSPQDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLFTTVGNDATFQPNPNSVYQTEHLSYFKFVGRMVAKALFDGQLLDVYFTRSFYKHILGVKVTYHDIEAVDPDYYKNLKWLLENDVSDILDLTFSMDADEEKHILYEKTEVTDYELKPGGRNIRVTEETKHEYVDLVADHILTSAIRPQINAFLEGLNELIPRELVSIFNDKELELLISGLPEIDFDDLKANTEYTSYTVGSPVIRWFWEVVKAFSKEDMARFLQFVTGTSKVPLEGFKALQGISGPQRLQIHKAYGSPERLPSAHTCFNQLDLPEYQSKEQVQERLLLAIHEANEGFGFA

ID	PTM Type	Color
ph	Phosphorylation	X
sno	S-nitrosylation	X
ox	Reversible Cysteine Oxidation	X
ub	Ubiquitination	X
so	S-sulfenylation	X
	Multiple types	X

Domains & Sites

Clear highlighted range

**Interpro Domains**
IPR ID	Description	From	To
IPR000569	HECT domain	3296	3658
IPR010309	E3 ubiquitin ligase, domain of unknown function DUF908	86	164
IPR010309	E3 ubiquitin ligase, domain of unknown function DUF908	201	356
IPR010314	E3 ubiquitin ligase, domain of unknown function DUF913	416	764
IPR015940	Ubiquitin-associated domain	1271	1312
IPR025527	HUWE1/REV1, ubiquitin-binding motif	2586	2616
		2626	2656
		2662	2693

**Sites**
Show	Type	Description	Position
	Site	Glycyl thioester intermediate	3625

AT1G70320.1

Arabidopsis thaliana [ath]

ubiquitin-protein ligase 2

54 PTM sites : 5 PTM types

PLAZA: AT1G70320

Gene Family: HOM05D000747

Other Names: UPL2

Uniprot

Q8H0T4

Link out to other resources with this protein ID : TAIR | PeptideAtlas | ARAPORT | PhosPhAt

PTMs

Sequence

Length: 3658

Domains & Sites

BLAST

Perform a BLAST search for this sequence, or a part of this sequence (minimum 50 characters)
A downloadable tutorial can be found here

AT1G70320.1

Arabidopsis thaliana [ath]

ubiquitin-protein ligase 2

54 PTM sites : 5 PTM types

PLAZA: AT1G70320

Gene Family: HOM05D000747

Other Names: UPL2

Uniprot

Q8H0T4

Link out to other resources with this protein ID : TAIR | PeptideAtlas | ARAPORT | PhosPhAt

PTMs

Sequence

Length: 3658

Domains & Sites

BLAST

Perform a BLAST search for this sequence, or a part of this sequence (minimum 50 characters)A downloadable tutorial can be found here

Perform a BLAST search for this sequence, or a part of this sequence (minimum 50 characters)
A downloadable tutorial can be found here